Improved Protocol for the Preparation of Tetraselmis suecica Axenic Culture and Adaptation to Heterotrophic Cultivation

نویسندگان

  • Mojtaba Azma
  • Rosfarizan Mohamad
  • Raha Abdul Rahim
  • Arbakariya B. Ariff
چکیده

The effectiveness of various physical and chemical methods for the removal of contaminants from the microalgae, Tetraselmis suecica, culture was investigated. The information obtained was used as the basis for the development of improved protocol for the preparation of axenic culture to be adapted to heterotrophic cultivation. Repeated centrifugation and rinsing effectively removed the free bacterial contaminants from the microalgae culture while sonication helped to loosen up the tightly attached bacterial contaminants on the microalgae cells. Removal of bacterial spores was accomplished using a mixture of two antibiotics, 5 mg/mL vancomycine and 10 mg/mL neomycine. Walne medium formulation with natural seawater was preferred for the enhancement of growth of T. suecica. Adaptation of growth from photoautotrophic to heterotrophic conditions was achieved by the repeated cultivation of photoautotrophic culture with sequential reduction in illumination time, and finally the culture was inoculated into the medium containing 10 g/L glucose, incubated in total darkness to obtain heterotrophic cells. Changes in the morphology and composition of T. suecica cells during the adaptation from photoautotrophic to heterotrophic condition, as examined under Transmission Electron Microscope, were also reported.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The growth, survival rate and reproductive characteristics of Artemia urmiana fed by Dunaliella tertiolecta, Tetraselmis suecica, Nannochloropsis oculata, Chaetoceros sp., Chlorella sp. and Spirolina sp. as feeding microalgae

This study was performed to compare the efficiency of six microalgae namely Dunaliella tertiolecta, Tetraselmis suecica, Nannochloropsis oculata, Chaetoceros sp < em>., Chlorella sp. and Spirolina sp < em>. on the growth, survival rate and reproduction efficacy in Artemia urmiana in laboratory conditions. Artemia cysts were harvested from Urmia Lake and hatched according to the standard method....

متن کامل

Control of Vibrio spp. in Shrimp Hatcheries Using the Green Algae Tetraselmis suecica

The Vibrio spp. are the most common and harmful shrimp pathogenic bacteria causing serious diseases of larval and postlarval stages in the hatchery. The Vibrio inhibitory activity of green algae Tetraselmis suecica has been reported in vitro (Austin and Day 1990).In these experiments, the inhibitory activity was assessed in vivo in a commercial shrimp hatchery of the white prawn Fenneropenaeus ...

متن کامل

The growth, survival rate and reproductive characteristics of Artemia urmiana fed by Dunaliella tertiolecta, Tetraselmis suecica, Nannochloropsis oculata, Chaetoceros sp., Chlorella sp. and Spirolina sp. as feeding microalgae

This study was performed to compare the efficiency of six microalgae namely Dunaliella tertiolecta, Tetraselmis suecica, Nannochloropsis oculata, Chaetoceros sp., Chlorella sp. and Spirolina sp. on the growth, survival rate and reproduction efficacy in Artemia urmiana in laboratory conditions. Artemia cysts were harvested from Urmia Lake and hatched according to the standard method. Live microa...

متن کامل

Prolonged Laboratory Maintenance of Toxoplasma gondii Tachyzoites in Co-cultivation with the Bovine Theileria annulata-Infected Lymphoblastoids

Background and Aims: In most of the studies, Toxoplasma gondii is maintained in laboratory mice or studied in vitro using non-lymphoid cell lines or primary mouse macrophages. The target of our research was to design a new axenic culture of Toxoplasma gondii tachyzoites to providing a sufficient quantity of them. Material and Methods: Theileria annulata-infected lymphoblastoids, which had been...

متن کامل

The independent effects of ferrous and phosphorus on growth and development of Tetraselmis suecica; an in vitro study

Five treatments including Conway medium, media containing 0.1, 0.17, 0.3 and 0.5 mg l-1 ferrous (Fe; in the first experiment), media with 1, 1.26, 1.59, 2 mg l-1 concentrations of phosphorous (P; in the second experiment) and a pure sample of Tetraselmis suecica were cultured under laboratory conditions. Growth rate of the algae was determined using the optical density method at 750 nm and numb...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2010